You've successfully activated your Lifescience account. You may now login with the following ID :. The revised versions of these documents can be found at the links provided. To order these products, please contact your Roche order management team. The kit is ideal for fast hot start PCR assays for gene detection and gene quantification. Online Technical Support Find help.
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It comes with a novel thermal cycler module tailored for heating and cooling miniaturized qPCRs in a multiwell plate with individual reaction wells. This makes optical read-out as specific as possible for chemical detection formats, while reducing the overall complexity of experimental layouts in a high-throughput scenario. The basic software module of the new system allows users to set up and run reaction protocols. The plate consists of two components: a thermally conductive unit containing well-like structures for the reaction liquid and an insulating top layer that prevents the heated lid of the instrument from affecting the analysis.
An important trend in biological and biochemical analysis over the past 15 years has been miniaturization and parallelization of analytical procedures. Large-scale, high-throughput analysis of gene expression or genetic polymorphisms is an essential element of modern functional genomics, where individual samples are screened against many thousands of target genes. Its high sensitivity make qPCR the well-established gold standard for precisely profiling gene expression levels or genotyping known SNPs.
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Evaluation of the LightCycler 1536 Instrument for High-Throughput Quantitative Real-Time PCR
You've successfully activated your Lifescience account. You may now login with the following ID :. The revised versions of these documents can be found at the links provided. Rapid, high-throughput, plate-based real-time PCR amplification and detection instrument. For life science research only. Not for use in diagnostic procedures. To order these products, please contact your Roche order management team.
High-throughput analysis using LightCycler® 1536 Real-Time PCR System
Quantitative real-time PCR qPCR is a frequently used, sensitive and accurate method to study gene expression profiles. However, its throughput was so far limited for routine laboratories to reactions per run based on the limitations of the available instruments. Recently, the LightCycler Instrument was launched providing a high-throughput solution for qPCR with the analysis of reactions in approximately 45 min. We assessed the accuracy and sensitivity of this novel technology for the analysis of gene expression profiles in combination with the Innovadyne Nanodrop Express pipetting robot.