We'd like to understand how you use our websites in order to improve them. Register your interest. The indirect immunoperoxidase and immunofluorescent antibody techniques were compared for their ability to detect antibodies to malignant catarrhal fever virus in experimentally and naturally infected cattle. The immunoperoxidase test detected titres of antibody 8-fold higher than those detected by immunofluorescence.
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We'd like to understand how you use our websites in order to improve them. Register your interest. Vero cells were compared with primary bovine thyroid BTh cultures for the isolation of malignant catarrhal fever virus from infected blood and tissues. Comparative titrations showed Vero cells detected only two-fold less infectivity in rabbit spleen suspensions than BTh cells.
Twenty three of 32 bovine buffy coat cell preparations which were positive on BTh cells were all also positive on Vero cells. The cytopathic effects CPE of virus isolates in Vero cells consisted of syncytia and refractile cytomegalic cells which were as easy to recognise and developed as rapidly as CPE in BTh cells.
Two laboratory strains of malignant catarrhal fever virus were readily adapted to and maintained by passage in Vero cells. This is a preview of subscription content, log in to check access. Castro, A. American Journal of Veterinary Research , 43 , 5— Edington, N. European Journal of Cancer , 15 , — Heuschele, W. Mushi, E. Bulletin of Animal Health and Production in Africa , 33 , 29— Google Scholar. Plowright, W. Research in Veterinary Science , 2 , — Nature , , — Research in Veterinary Science , 19 , — Journal of General Microbiology , 39 , — Download references.
Reprints and Permissions. Rossiter, P. Use of vero cells for the isolation and propagation of malignant catarrhal fever virus. Trop Anim Health Prod 19, 77—81 Download citation. Accepted : 15 August Issue Date : June Search SpringerLink Search. Summary Vero cells were compared with primary bovine thyroid BTh cultures for the isolation of malignant catarrhal fever virus from infected blood and tissues.
References Castro, A. Gumm Authors P. Rossiter View author publications. You can also search for this author in PubMed Google Scholar. View author publications. Rights and permissions Reprints and Permissions. About this article Cite this article Rossiter, P.
Use of vero cells for the isolation and propagation of malignant catarrhal fever virus