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Toggle navigation. Have you forgotten your login? Poster communications. Kocaman 1 Y. Izgu 3 Reyad Elsharkawy 2 Details. Kocaman 1 AuthorId : Author. Yalcin Mendi 1 AuthorId : Author. Izgu 3 AuthorId : Author. Reyad Elsharkawy 2 AuthorId : Author. Hide details. Abstract : Alcohol dehydrogenases ADH participate the biosynthetic pathway of aroma volatiles in fruit by interconverting aldehydes to alcohols and providing substrates for the formation of esters. Different regeneration media were investigated to improve transformation efficiency.
The best regeneration result was obtained from MS medium supplemented with 2. Regeneration protocol has been applied to obtain transformed plants, using Agrobacterium tutriefaderis which has a vector RNAi pFGC bp carrying genes of resistance to kanamycifie and Bar. Transformed plants were characterized by PCR analysis. Keywords : 'Vedrantais' alcohol dehydrogenases RNAi regeneration aldehyde reductase.
Citation E. Kocaman, Y. Izgu, Reyad Elsharkawy. Transformation of Cm-ADH gene to melon genotype. Metrics Record views. Contact support.
Solvay mise sur la chimie durable issue de la biomasse et choisit le Brésil
Toggle navigation. Have you forgotten your login? Poster communications. Kocaman 1 Y. Izgu 3 Reyad Elsharkawy 2 Details.
[Aromas and biotechnology]. [French] 
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Production d'arômes de type lactone par des levures 
Lipoxygenase enzymatic pathway is a widely studied mechanism in the plant kingdom. Combined actions of three enzymes: lipase, lipoxygenase LOX and hydroperoxide lyase HPL convert lipidic substrates such as C and C fatty acids into short chain volatiles. These reactions, triggered by cell membrane disruptions, produce compounds known as Green Leaf Volatiles GLVs which are C 6 or C 9 -aldehydes and alcohols. These GLVs are commonly used as flavors to confer a fresh green odor of vegetable to food products. Therefore, competitive biocatalytic productions have been developed to meet the high demand in these natural flavors. However this second step of the bioconversion presents low yield due to the HPL instability and the inhibition by its substrate.
Transformation of Cm-ADH gene to melon genotype