7451 DATASHEET PDF

JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser. The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody.

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JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser. The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization.

This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. Oncogenesis 6, e; doi We showed that our reagents are suitable for IHC detection on each of these platforms. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation.

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10 and greater sensitivity than other polymer systems. The staining procedure is simple as shown in the diagram below.

Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody.

Sections are again rinsed and the slides are developed with the peroxidase substrate of choice. Product review on Biocompare. How do I Request a Quote or Bulk pricing? Skip to the end of the images gallery. Skip to the beginning of the images gallery. Product FAQs. Related Products. Technical Information. Description The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system.

Our research lab has an open automated staining platform. Do you know if the ImmPRESS reagents can be applied to an automated system, and if so, do you have any guidelines or procedures? Normal Goat Serum Blocking Solution, 2. Citations Powered by Bioz See more details on Bioz. Technical Information The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones.

Staining Procedure The staining procedure is simple as shown in the diagram below. Consider Species Cross-Reactivity When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue.

If the species of the primary antibody and the species of the tissue are closely related e. The following options minimize background staining in these instances: Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species e. Use the M. MP for applications of mouse primary antibodies on mouse tissue.

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